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Laccase;
CAS No.: 80498-15-3;
EC No.: 1.10.3.2;
EINECS: 420-150-4.
Applications:
Textile;
Paper;
Food;
Water treatment.
Appearance: Liquid (for food), power (for industry)
pH: 3.0-5.5;
Temperature: 20-60ºC;
Laccases (EC 1.10.3.2) are copper-containing oxidase enzymes that are found in many plants, fungi, and microorganisms. The copper is bound in several sites; Type 1, Type 2, and/or Type 3. The ensemble of types 2 and 3 copper is called a trinuclear cluster. Type 1 copper is available to action of solvents, such as water. It can be displaced by mercury, substituted by cobalt or removed via a copper complexone. Removal of type 1 copper causes a decrease in laccase activity. Cyanide can remove all copper from the enzyme however re-embedding with type 1 and type 2 copper has been shown to be impossible.
Type 3 copper however can be embed back into the enzyme. Laccases act on phenols and similar molecules, performing a one-electron oxidations, which remain poorly defined. It is proposed that laccases play a role in the formation of lignin by promoting the oxidative coupling of monolignols, a family of naturally occurring phenols. Laccases can be polymeric, and the enzymatically active form can be a dimer or trimer. Other laccases, such as ones produced by the fungus Pleurotus ostreatus, play a role in the degradation of lignin, and can therefore be included in the broad category of ligninases.
Because laccase belongs to the oxidase enzyme family it requires oxygen as a second substrate for the enzymatic action.
Laccase is a highly active enzyme that made from Aspergillus Oryzae. It can catalyze phenols and its derivatives.
Laccase (Laccase E. C. 1.10.3.2) is a containing copper. It can catalyze phenols and its derivatives, aromatic amine and its derivatives, carboxylic acids and its derivatives, steroid hormone, biochrome, organometallic compounds and non-phenols substrate.
1. For indigo-dye-fading technique of jean processing by using laccase and catalysis enzymes in jean-washing industry,
2. For selectively catalyze lignin-degradation and pulp bleaching by using laccase combined medium and xylanase. It is also a new environment friendly technique in wastepaper deinking process.
3. For chlorophenols organic compounds degradation of wastewater treatment (which in line with pH requirements of laccase).
4. For baking.
5. For extract sugar. It can raise color value remaining.
6. For others using as fiberboard adhesive, hair dyeing, lacquer dyeing film formation, crosslinking agent and biological measurement.
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